{"id":940,"date":"2020-01-30T11:09:56","date_gmt":"2020-01-30T10:09:56","guid":{"rendered":"http:\/\/sagoebel.de\/UngermannLab\/Version_B\/?p=940"},"modified":"2020-08-23T20:26:09","modified_gmt":"2020-08-23T18:26:09","slug":"a-research-2","status":"publish","type":"post","link":"https:\/\/www.biochemie.uni-osnabrueck.de\/?p=940","title":{"rendered":"Endosome maturation"},"content":{"rendered":"\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"has-text-align-center wp-block-heading\">  Endosome maturation <\/h2>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"608\" height=\"340\" src=\"https:\/\/www.biochemie.uni-osnabrueck.de\/wp-content\/uploads\/2019\/11\/Screen-Shot-2018-10-16-at-12.51.26-.jpg\" alt=\"\" class=\"wp-image-633\" srcset=\"https:\/\/www.biochemie.uni-osnabrueck.de\/wp-content\/uploads\/2019\/11\/Screen-Shot-2018-10-16-at-12.51.26-.jpg 608w, https:\/\/www.biochemie.uni-osnabrueck.de\/wp-content\/uploads\/2019\/11\/Screen-Shot-2018-10-16-at-12.51.26--300x168.jpg 300w, https:\/\/www.biochemie.uni-osnabrueck.de\/wp-content\/uploads\/2019\/11\/Screen-Shot-2018-10-16-at-12.51.26--150x84.jpg 150w\" sizes=\"auto, (max-width: 608px) 100vw, 608px\" \/><\/figure><\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"sauron_divider\">\n            <span class=\"div_left\"><\/span>\n    <span class=\"div_middle\">\n      <i class=\"fa fa-stop\"><\/i>\n    <\/span>\n            <span class=\"div_right\"><\/span>\n          <\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p><\/p>\n\n\n\n<p>Endosomes are organelles that are localized at the branch point between the plasma membrane and the vacuole. Early endosomes fuse with endocytic vesicles and then mature into late endosomes. These then fuse with the vacuole in yeast (or lysosome in metazoan cells). At endosomes, some membrane proteins are sorted into intraluminal vesicles for subsequent degradation inside vacuole, whereas others are sorted into tubules and transported back to the plasma membrane. The time-course of such cargo transport to the vacuole is seen in the images below. <\/p>\n\n\n\n<p>We study how the fusion machinery is recruited and activated to make this possible. In particular, we are interested in the involved Rab GTPases, Rab5 (or Vps21) and Rab7 (or Ypt7), their GEFs (in particular the Mon1-Ccz1 complex) and the tethering complexes HOPS and CORVET. We use both live-cell imaging and reconstitution in the test tube to dissect this process. <\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"683\" height=\"276\" src=\"https:\/\/www.biochemie.uni-osnabrueck.de\/wp-content\/uploads\/2019\/11\/Screen-Shot-2018-10-16-at-12.34.31-.jpg\" alt=\"\" class=\"wp-image-626\" srcset=\"https:\/\/www.biochemie.uni-osnabrueck.de\/wp-content\/uploads\/2019\/11\/Screen-Shot-2018-10-16-at-12.34.31-.jpg 683w, https:\/\/www.biochemie.uni-osnabrueck.de\/wp-content\/uploads\/2019\/11\/Screen-Shot-2018-10-16-at-12.34.31--300x121.jpg 300w, https:\/\/www.biochemie.uni-osnabrueck.de\/wp-content\/uploads\/2019\/11\/Screen-Shot-2018-10-16-at-12.34.31--150x61.jpg 150w\" sizes=\"auto, (max-width: 683px) 100vw, 683px\" \/><\/figure><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Endosome maturation Endosomes are organelles that are localized at the branch point between the plasma membrane and the vacuole. Early endosomes fuse with endocytic vesicles and then mature into late endosomes. These then fuse with the vacuole in yeast (or lysosome in metazoan cells). At endosomes, some membrane proteins are sorted into intraluminal vesicles for&#8230;<\/p>\n","protected":false},"author":10,"featured_media":187,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,2],"tags":[],"class_list":["post-940","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-allgemein","category-bilder"],"_links":{"self":[{"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=\/wp\/v2\/posts\/940","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=940"}],"version-history":[{"count":19,"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=\/wp\/v2\/posts\/940\/revisions"}],"predecessor-version":[{"id":1605,"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=\/wp\/v2\/posts\/940\/revisions\/1605"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=\/wp\/v2\/media\/187"}],"wp:attachment":[{"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=940"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=940"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biochemie.uni-osnabrueck.de\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=940"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}